Actin Staining

So this morning I looked at the slides I stained and had a mini breakdown. I couldn't find any cells, anywhere! Luckily, Katherine took a look, and found some. I'm such an idiot... not sure what I was doing wrong.

Anyway, here a couple of the pictures I took. The blue is DAPI, showing the nucleus of the human airway smooth muscle cells. The red and green, TRITC and FITC respectively, stain the actin. The pictures are a lot clearer in the Nikon software - you can see individual strands / thread of the red actin throughout the cells.

Now we have to try and figure out how to assess and compare polarity.